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pet28a lbcas12a  (Addgene inc)


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    Structured Review

    Addgene inc pet28a lbcas12a
    Pet28a Lbcas12a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+lbcas12a/pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis+(RTW645)+(Plasmid+%23114070)/pmc12563427-33-8-9
    Average 88 stars, based on 2 article reviews
    pet28a lbcas12a - by Bioz Stars, 2026-10
    88/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients
    Article Snippet: .. Escherichia coli BL21 (DE3) cells were transformed with pET28a–LbCas12a (addgene No. #114070) bacterial expression vectors to express LbCas12a recombinant protein. .. The cells were cultured in Luria Broth (LB) at 37 °C until the culture reached an O.D of 0.6, and the recombinant protein was induced at 18 °C for 18 h by the addition of isopropyl β-D-thiogalactoside (IPTG, GenDEPOT, Katy, TX, USA) to a final concentration of 1 mM.

    Expressing:

    Article Title: CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients
    Article Snippet: .. Escherichia coli BL21 (DE3) cells were transformed with pET28a–LbCas12a (addgene No. #114070) bacterial expression vectors to express LbCas12a recombinant protein. .. The cells were cultured in Luria Broth (LB) at 37 °C until the culture reached an O.D of 0.6, and the recombinant protein was induced at 18 °C for 18 h by the addition of isopropyl β-D-thiogalactoside (IPTG, GenDEPOT, Katy, TX, USA) to a final concentration of 1 mM.

    Recombinant:

    Article Title: CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients
    Article Snippet: .. Escherichia coli BL21 (DE3) cells were transformed with pET28a–LbCas12a (addgene No. #114070) bacterial expression vectors to express LbCas12a recombinant protein. .. The cells were cultured in Luria Broth (LB) at 37 °C until the culture reached an O.D of 0.6, and the recombinant protein was induced at 18 °C for 18 h by the addition of isopropyl β-D-thiogalactoside (IPTG, GenDEPOT, Katy, TX, USA) to a final concentration of 1 mM.



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